Reliable storage of Ureaplasma and Mycoplasma

SIGMA VCM™ proven to be reliable at storing and transporting Ureaplasma and Mycoplasma

A common sexually transmitted infection, Ureaplasma, can come in two species, Ureaplasma parvum and Ureaplasma urealyticum, as well as multiple serovars. Infection with Ureaplasma can result in premature births, making its effective diagnosis and treatment imperative. Given the delicate nature of Ureaplasma, the effective transport of clinical samples so that viability of the bacteria is maintained is an essential part of an effective diagnosis.

The Clinical and Laboratory Standards Institute Standard M40-A2 gives a minimum basic requirement of 48 hours for bacteria to survive in transport devices at ambient or refrigerated temperatures.

A study carried out by Rees and Spiller at 4 different temperatures over 264 hours, to ensure they meet the basic requirements and to study how Ureaplasma copes with different conditions and freeze-thaw cycles.

Similarly, Mycoplasma hominis, a notoriously fastidious organism, is sensitive to environmental factors. Transport medium and conditions are important to successful detection by culture in
clinical assays. Rees and Spiller did another study to evaluate the stability of M. hominis in Sigma-VCM™ universal transport device, at different storage temperatures over 192 hours.

Both these studies showed that Viability of Ureaplasma species in Ʃ-VCM transport medium was stable for 4 days at room temperature. Viability could be extended to at least 264 hours if kept at 4˚C. In one study, despite 8 freeze-thaw cycles from -80oC to RT, 3 of the 4 strains maintained near baseline viability. This feature of Sigma-VCM™ provides a significant improvement on currents methods of laboratory storage of M. hominis. They also showed that Ʃ-VCM transport medium has met and exceeded the basic requirements for transport of bacteria set out by the Clinical and Laboratory Standards Institute Standard M40-A2, and extended viability is possible through freeze-thaw cycles.

Share this page

Related Clinical studies

Adenovirus – What do we know and what is new?

Recently, the World Health Organisation (WHO) released a report on acute hepatitis in children with an unknown origin. Even though Adenovirus is a hypothesis as being one of the strongly associated reasons for Hepatitis in this report, it is still under investigation. United Kingdom is one of the highly affected countries. PCR analysis has been the first line of diagnosis. Blood sampling, respiratory sampling and stool sampling are being used to collect specimen. Respiratory specimen in a VTM such as the ∑-Virocult® (Viral transport media) and ∑-MM™ inactivation media can be used to collect samples.

Read More »

Polio – what can we do to prevent it?

Poliovirus can be cultured from faeces which usually goes through a prolonged cell culture process. MWE’s Faecal Transwab® compatible with PCR, would be a suitable medium to collect and transport the sample to the laboratory if faecal sample was not readily available or desirable.

Read More »

Contact us

Have a question or would like to find out about one of our products? Please fill in the form below and we’ll be in touch soon.

Contact Popup

Product enquiry

For buying enquiries, or for more information about this product, please fill in the form below and we’ll be in touch soon.

Product enquiry

Let’s see...

We can direct you to the best place. Would you like help with:

Need help?
Choice