Recovery of Genomic DNA and Isolation of 16S Ribosomal DNA from SIGMA-TRANSWAB® System with Viability Acceptance in Accordance with CLSI M40-A2 Standard

SIGMA-TRANSWAB® liquid Amies paired with foam and flock swabs demonstrated compliant recovery of a broad range of aerobic, anaerobic, and fastidious organisms under CLSI M40-A2 conditions. Across ten bacterial species, including both Gram-positive and Gram-negative pathogens, both foam and flocked swabs met viability and overgrowth criteria at room temperature and refrigerated storage, with successful recovery confirmed by culture and 16S rDNA PCR.